HDDC Organoid Core

As part of the Breault Lab, the HDDC Organoid Core provides training and protocols to collaborators wishing to move research towards
in vitro gastrointestinal organoid culture.

Primary Contact

Dr. David Breault, Principle Investigator
david.breault@childrens.harvard.edu
617-919-2930

Core Research Assistant

Wanshu Qi, PhD
wanshu.qi@childrens.harvard.edu
Center for Life Sciences 16th Floor, 16030.17

Core Director

Eric Stas, MS
eric.stas@childrens.harvard.edu
617-355-3046
Center for Life Sciences 16th Floor, 16030.16

Core Research Assistant

Prabhath Mannamn
prabhath.mannam@childrens.harvard.edu
Center for Life Sciences 16th Floor, 16030.18

Reagents Available for Purchase (iLab Link)

Noggin/R-Spondin-1/Wnt3a Conditioned Media
R-Spondin-1 Conditioned Media
Corning Matrigelâ„¢ Basement Membrane Matrix
Recombinant Murine Noggin

Training and Services Available

Murine Gastrointestinal Crypt Isolation and Organoid Culture
Human Gastrointestinal Crypt Isolation and Organoid Culture
Immunofluorescence Staining & Imaging Training
Organoid Experimental Design Consulting
Imaging with Invitrogen EVOS FL Auto 2