HDDC Organoid Core

As part of the Breault Lab, the HDDC Organoid Core provides training and protocols to collaborators
wishing to move research towards in vitro gastrointestinal organoid culture.

Primary Contacts
Dr. David Breault, Principle Investigator
david.breault@childrens.harvard.edu
617-919-2930

Research Assistants

Eric Stas
eric.stas@childrens.harvard.edu
617-355-3046
Center for Life Sciences 16th Floor, 16030.16
Elizabeth Howe Vera
elizabeth.howevera@childrens.harvard.edu
617-355-6000
Center for Life Sciences 16th Floor, 16030.17
Reagents Purchasable through iLab Training & Services
Noggin/R-Spondin-1/Wnt3a Conditioned Media
R-Spondin-1 Conditioned Media
Corning Matrigel™ Basement Membrane Matrix
Recombinant Murine Noggin
Murine Gastrointestinal Crypt Isolation
Murine Gastrointestinal Organoid Culture
Human Gastrointestinal Organoid Culture
Immunofluorescence Staining & Imaging Training
Organoid Experimental Design Consulting
Imaging with Invitrogen EVOS FL Auto 2

Protocols
Murine Gastrointestinal Media Components
Murine Gastrointestinal Crypt Isolation & Maintenance